ISSN Online: 2688-7231
ISBN Online: 978-1-56700-478-6
Proceedings of the 24th National and 2nd International ISHMT-ASTFE Heat and Mass Transfer Conference (IHMTC-2017)
THERMAL STUDY OF LAB ON CHIP (LOC) SYSTEM FOR PCR REACTION APPLICATIONS
Abstract
In this paper, the thermal study of
Polydimethylsiloxane (PDMS) based lab on chip
(LoC) system on a glass substrate for polymerase
chain reaction (PCR) has been presented. The system
consist of a microfluidic structure on one side of 5×5
cm2 of glass substrate comprises of a microchannel
channel with inlet and outlet holes for the fluid
handling, a reaction chamber for the thermal cycle of
polymerase chain reaction (PCR), and two thermally
actuated membrane-type microvalves to confine the
water based biological solution. While on the other
side of glass substrate three thin film Cr/Al/Cr metal
heaters have been integrated. Two lateral heaters,
located under the valves near the inlet and outlet
positions of the chamber, actuate the valves and allow
the chamber isolation, while the last one, positioned
below the chamber, is dedicated to the PCR thermal
cycle. In order to optimize heaters and valves
geometry, the temperature distribution over the
heaters and the membrane of valve deformation have
been studied using the commercial software COMSOL
Multiphysics. The heaters have been fabricated on
glass substrate according to simulated structures
using soft lithography. In order to check the reliability
of structure, thermal study of fabricated heaters have
been performed using FLIR IR camera, which shows a
good agreement with simulated values.