This study presents a comprehensive investigation into the performance and operational characteristics of a single-loop pulsating heat pipe (PHP) under asymmetric heating conditions. Utilizing a validated computational fluid dynamics (CFD) model, numerical simulations are conducted to analyze the thermal performance, flow patterns, and heat transfer characteristics of the PHP. The experimental setup involves a single-loop PHP with an asymmetric diameter configuration, which facilitates enhanced heat transfer and control over fluid flow. The PHP is subjected to varying heat inputs and operating conditions to systematically explore its behavior. The findings of this study reveal that the application of asymmetric heating significantly influences the PHP's operation, leading to notable variations in flow patterns and temperature distribution along the condenser and evaporator sections. The asymmetric heating profile proves beneficial as it promotes intensified fluid circulation and effectively mitigates temperature imbalances within the system. By employing CFD simulations, a detailed understanding of the fluid behavior, temperature distribution, start-up time, and thermal resistance of the PHP under asymmetric heating conditions is achieved. This comprehensive analysis sheds light on the intricate interplay between asymmetric heating and its impact on the overall thermal efficiency of the PHP.