ISSN Online: 2688-7231
ISBN Online: 978-1-56700-524-0
Proceedings of the 26thNational and 4th International ISHMT-ASTFE Heat and Mass Transfer Conference December 17-20, 2021, IIT Madras, Chennai-600036, Tamil Nadu, India
Development of a numerical model for cell shrinkage during cryopreservation of cells
Resumo
Cryopreservation is a process which preserves cells, tissues, and other biological samples by cooling the samples to
very low temperatures along with the use of cryoprotecting
agents (CPA). In this paper, a computational model for
simulating the transport of water from the intracellular to the
extracellular water-CPA solution during cryopreservation of
cells is presented. The main novelty of the study is that the
effect of water transport on the shrinkage of the cells is
captured. The developed model is used to study the effect of
different parameters such as the number of cells, cell size and
different CPA concentration on diffusion pattern and
shrinkage for a domain containing single or multiple cells. It is found that the shrinkage percentage is higher if the cell size is smaller, if less number of cells is present in the domain, and if the water concentration in the extracellular region is lower.